بررسی نرخ بقا تخمک پس از فریز دو روش slow freezing و vitrification

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مقایسه وقوع آپوپتوز، بقا و بلوغ در فولیکول های پره آنترال موش بعد از slow freezing و cryotop vitrification

این پژوهش به منظور ارزیابی اثر انجماد شیشه ای کرایوتاپ و انجماد آهسته بر میزان بقا، بلوغ و بیان ژنهای آپوپتوز در فولیکول های پره آنترال جداشده از تخمدان موش انجام گرفت. مطالعه به روش تجربی بر روی موش های نابالغ 12- 14 روزه نژاد nmri انجام شد. فولیکول های پره آنترال جدا شده به گروه های غیرانجمادی، انجماد شیشه ای و انجماد آهسته تقسیم شدند. میزان بقا در فولیکول های گروه های غیرانجمادی، انجماد شی...

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Slow freezing, vitrification and ultra-rapid freezing of human embryos: a systematic review and meta-analysis.

Embryo cryopreservation is an important aspect of assisted reproduction. Many methods have been described, but they have been poorly investigated in randomized trials, highlighting the need for a systematic review of the literature. Meticulous electronic/hand searches were performed to locate randomized trials (RCT) comparing embryo cryopreservation methods. Primary outcomes were clinical pregn...

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In vitro survival of in vitro-produced bovine embryos cryopreserved by slow freezing, fast freezing and vitrification

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The ability to spur growth of early stage gametic cells recovered from neonates could lead to significant advances in rescuing the genomes of rare genotypes or endangered species that die unexpectedly. The purpose of this study was to determine, for the first time, the ability of two substantially different cryopreservation approaches, slow freezing versus vitrification, to preserve testicular ...

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Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled-rate -1°C/minute cell freezing in a -80°C freezer and tested four cryoprotective agents (CPA), namely dimethyl sulfoxide (DMSO), ethylene glycol (...

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